| Congresso Brasileiro de Microbiologia 2023 | Resumo: 707-1 | ||||
Resumo:Anaplasmosis is a disease caused by bacteria of the genus Anaplasma, transmitted by ticks of the Ixodidae family. The most frequent species in dogs are A. platys and A. phagocytophilum, which are also described as pathogenic for humans. Given the importance of this pathogen for public health and the important role of dogs in transmission to humans, the aim of this study was to determine the prevalence of these pathogens in dogs from a shelter named Parque Francisco de Assis in the municipality of Lavras, Minas Gerais-Brazil. A longitudinal study was carried out in which two samplings with an interval of six months were performed, characterizing two prevalence panels. The first sample collection was carried out in the dry season (July/August 2019) and the second in the rainy season (January/February 2020). The entire resident canine population was microchipped and evaluated through clinical examination, performed by veterinarians. Whole blood and serum samples were also collected and used to perform blood count, blood smear, Polymerase Chain Reaction (PCR-Nested) and serological tests. Reactive sera with titers ≥ 320 were considered positive for A. phagocytophilum. All blood samples were subjected to DNA extraction using the PureLink® Genomic DNA Kit (Invitrogen™, Thermo Fisher Scientific, USA). The search for A. phagocytophilum DNA was performed by amplification of the msp4 gene. There were 329 and 310 animals living in Parque Francisco de Assis in the first and second samplins, respectively. The serological tests performed indicated 4.56% (15/329) and 9.36% (29/310) of animals having anti-A. phagocytophilum antibodies, in the first and second samples, respectively. The clinical and hematological evaluation of these seropositive animals showed the following most relevant findings in the first and second sampling respectively: lymphadenopathy 66.67% (10/15) and 34.48% (10/29); skin disorders (lesion or alopecia) 53.33% (8/15) and 13.79% (4/29); fever 40% (6/15) and 24.14% (7/29); thrombocytopenia 40.00% (6/15) and 44.83% (13/29), and anemia 13.33% (2/15) and 13.79% (4/29). The DNA of A. phagocytophilum was not detected in any of the analyzed samples in both collections. Intraplatelet inclusions suggestive of A. platys were observed in 5.78% (18/329) and 4.83% (15/310), in the first and second sampling respectively. The most relevant clinical and hematological findings in the positive animals in the first and second sampling, respectively, were: lymphadenopathy 16.67% (3/18) and 46.67% (7/15); skin disorders (lesion or alopecia) 22.22% (4/18) and 40.00% (6/15); temperature increase 38.89% (7/18) and 66.67% (1/15) and thrombocytopenia 38.89% (7/18) and 33.33% (5/15). The results of this study demonstrated a low prevalence of anaplasmosis in the canine population of the sampled shelter, with lymphadenopathy, skin lesions, temperature increase and thrombocytopenia being the most frequent clinical-hematological aspect observed in the positive animals. Palavras-chave: Zoonoses, One health, A. phagocytophilum, A. platys Agência de fomento:CAPES, FAPEMIG, CNPq | |||||